Here, we mixed an efficient methodology for protein extraction from tissue samples with lysine-acetylated peptide immunoprecipitation and high-accuracy tandem mass spectrometric (MS/MS) measurements, which allowed us to generate an atlas of lysine acetylation websites in sixteen totally different rat tissues.

The exception to the rule is the DOT1 family, members of which methylate K79 within the globular area of histone H3 and that are structurally not associated to SET-area proteins four - 6 Current work suggests that SET-domain-containing proteins methylate just a few proteins along with histones (see later)